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KMID : 0379120110390010031
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Korean Journal of Mycology
2011 Volume.39 No. 1 p.31 ~ p.38
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Development of Suhan Strain-specific SCAR Marker in Pleurotus ostreatus
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Seo Kyoung-In
Jang Kab-Yeul
Yoo Young-Bok
Park Soon-Young
Kim Kwang-Ho
Kong Won-Sik
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Abstract
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In this study, 81 commercial strains of Pleurotus species cultivated in South Korea were analyzed with randomly amplified polymorphic DNA (RAPD) technique. Sequence characterized amplified region (SCAR) markers were developed by designing from one RAPD polymorhic band specific to Suhan strain. The SCAR primer pair ‘S-OPA13-1’ amplified a 590-bp fragment in the varieties originated from Suhan strain. The Blast search of S-OPA13-1 showed high homology to the POMFBO1 P. ostreatus cDNA clone MFB02-A05 and Laccaria bicolor S238N-H82. The results showed that this SCAR marker can clearly distinguish Suhan strains from Pleurotus spp.
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KEYWORD
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Pleurotus ostreatus, RAPD, SCAR marker, Specific primers, Suhan strain
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